Chloramphenicol acetyltransferase in mammalian cells.
Recombinant genomes which categorical chloramphenicol acetyltransferase in mammalian cells.
We constructed a collection of recombinant genomes which directed expression of the enzyme chloramphenicol acetyltransferase (CAT) in mammalian cells.
The prototype recombinant on this collection, pSV2-cat, consisted of the beta-lactamase gene and origin of replication from pBR322 coupled to a simian virus 40 (SV40) early transcription area into which CAT coding sequences have been inserted.
Readily measured ranges of CAT amassed inside 48 h after the introduction of pSV2-cat DNA into African inexperienced monkey kidney CV-1 cells. As a result of endogenous CAT exercise just isn’t current in CV-1 or different mammalian cells, and since speedy, delicate assays for CAT exercise can be found, these recombinants offered a uniquely handy system for monitoring the expression of overseas DNAs in tissue traditioncells.
To exhibit the usefulness of this method, we constructed derivatives of pSV2-cat from which half or all the SV40 promoter area was eliminated.
Deletion of 1 copy of the 72-base-pair repeat sequence within the SV40 promoter brought about no important lower in CAT synthesis in monkey kidney CV-1 cells; nonetheless, a further deletion of 50 base pairs from the second copy of the repeats lowered CAT synthesis to 11% of its degree within the wild sort.
We additionally constructed a recombinant, pSV0-cat, by which your complete SV40 promoter area was eliminated and a distinctive HindIII web site was substituted for the insertion of different promoter sequences.
Description: A competitive ELISA for quantitative measurement of Monkey Caldesmon Kinase II in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Caldesmon Kinase II in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Caldesmon Kinase II in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey phosphorylated Caldesmon Kinase II in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey phosphorylated Caldesmon Kinase II ELISA kit
Description: A competitive ELISA for quantitative measurement of Monkey phosphorylated Caldesmon Kinase II in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey phosphorylated Caldesmon Kinase II ELISA kit
Description: A competitive ELISA for quantitative measurement of Monkey phosphorylated Caldesmon Kinase II in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Phosphorylated Caldesmon Kinase II ELISA Kit
Description: A sandwich ELISA kit for detection of Caldesmon from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: A sandwich ELISA kit for detection of Caldesmon from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: A competitive ELISA for quantitative measurement of Porcine Caldesmon in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Caldesmon in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Caldesmon in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Caldesmon in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Caldesmon in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Caldesmon in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Caldesmon in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Caldesmon in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Caldesmon in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Caldesmon in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Caldesmon in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Caldesmon in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
The epithelial-mesenchymal transition generates cells with properties of stem cells.
The epithelial-mesenchymal transition (EMT) is a key developmental program that’s typically activated throughout most cancers invasion and metastasis. We right here report that the induction of an EMT in immortalized human mammary epithelial cells (HMLEs) ends in the acquisition of mesenchymal traits and within the expression of stem-cell markers.
Moreover, we present that these cells have an elevated capability to type mammospheres, a property related to mammary epithelial stem cells.
Impartial of this, stem cell-like cells remoted from HMLE cultures type mammospheres and categorical markers just like these of HMLEs which have undergone an EMT.
Furthermore, stem-like cells remoted both from mouse or human mammary glands or mammary carcinomas categorical EMT markers. Lastly, remodeled human mammary epithelial cells which have undergone an EMT type mammospheres, tender agar colonies, and tumors extra effectively.
These findings illustrate a direct hyperlink between the EMT and the achieve of epithelial stem cellproperties.
Glioma stem cells promote radioresistance by preferential activation of the DNA injury response.
Ionizing radiation represents the best remedy for glioblastoma (World Well being Group grade IV glioma), one of the deadly human malignancies, however radiotherapy stays solely palliative due to radioresistance.
The mechanisms underlying tumour radioresistance have remained elusive. Right here we present that most cancers stem cellscontribute to glioma radioresistance by preferential activation of the DNA injury checkpoint response and a rise in DNA restore capability.
The fraction of tumour cells expressing CD133 (Prominin-1), a marker for each neural stem cells and mind most cancers stem cells, is enriched after radiation in gliomas.
In each celltradition and the brains of immunocompromised mice, CD133-expressing glioma cellssurvive ionizing radiation in elevated proportions relative to most tumour cells, which lack CD133. CD133-expressing tumour cellsremoted from each human glioma xenografts and first affected person glioblastoma specimens preferentially activate the DNA injury checkpoint in response to radiation, and restore radiation-induced DNA injury extra successfully than CD133-negative tumour cells.
As well as, the radioresistance of CD133-positive glioma stem cells may be reversed with a particular inhibitor of the Chk1 and Chk2 checkpoint kinases.
Our outcomes recommend that CD133-positive tumour cells characterize the cellular inhabitants that confers glioma radioresistance and may very well be the supply of tumour recurrence after radiation.
Concentrating on DNA injury checkpoint response in most cancers stem cells could overcome this radioresistance and supply a therapeutic mannequin for malignant mind cancers.
Description: Quantitativesandwich ELISA kit for measuring Rat Cathepsin L1 (CTSL1) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Rat Cathepsin L1(CTSL1) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativesandwich ELISA kit for measuring Mouse Cathepsin L1 (CTSL1) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Mouse Cathepsin L1(CTSL1) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
