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aged-associated macrophage irritation to enhance bone regeneration

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Maresin 1 resolves aged-associated macrophage irritation to enhance bone regeneration

 

Inflammaging is related to poor tissue regeneration noticed in superior age. Particularly, protracted irritation after acute damage has been related to decreased bone fracture therapeutic and elevated charges of nonunion in aged sufferers.
 Right here, we investigated the efficacy of utilizing Maresin 1 (MaR1), an omega-3 fatty acid-derived pro-resolving agent, to resolve irritation after tibial fracture damage and subsequently bettering aged bone therapeutic. Aged (24-month-old mice) underwent tibial fracture surgical procedure and had been both handled with car or MaR1 Three days after damage.
Fracture calluses had been harvested 7 days, 14 days, 21 days, and 28 days after damage to research inflammatory response, cartilage growth, bone deposition, and mechanical integrity, respectively. Therapeutic bones from MaR1-treated mice displayed decreased cartilage formation and elevated bone deposition which resulted in elevated structural stiffness and elevated pressure to fracture within the later levels of restore.
Within the early levels, MaR1 remedy decreased the variety of pro-inflammatory macrophages throughout the fracture callus and decreased the extent of inflammatory biomarkers in circulation. In tissue tradition fashions, MaR1 remedy of bone marrow-derived macrophages from aged mice protected cells kind a pro-inflammatory phenotype and induced an anti-inflammatory destiny.
Moreover, the secretome of MaR1-treated bone marrow-derived macrophages was recognized as osteoinductive, enhancing osteoblast differentiation of bone marrow stromal cells. Our findings right here establish decision of irritation, and MaR1 itself, to be a degree of intervention to enhance aged bone therapeutic.
Key phrases: bone; fracture therapeutic; inflammaging; macrophage; osteoblast.
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Human Normal Peripheral Blood CD3+ Pan T Cells
PBCD3-F100M 100 million Ask for price

Human Normal Peripheral Blood CD3+ Pan T Cells
PBCD3-F500M 500 million Ask for price

Human Normal Peripheral Blood CD3+ Pan T Cells
PBCD3-F50M 50 million
EUR 1798.8

T-Pro Stacking Buffer
JB03-C002 500ml/BT
EUR 182.4

T-Pro MycoClean spray
JT90-R002 500ml/BT
EUR 172.8

Rat Pan B Cells antibody
10R-B124a 50 ug
EUR 910.8
Description: Mouse monoclonal Rat Pan B Cells antibody

Human Peripheral Blood CD3+ T Cells, Fresh
PBCD3-F20M - Ask for price

T-Pro EZ Gel Solution 8%
JB02-B008M 500ml/BT
EUR 266.4

T-Pro EZ Gel Solution 8%
JB02-B008S 100ml/BT
EUR 162

T-Pro EZ Gel Solution 10%
JB02-B010M 500ml/BT
EUR 266.4

T-Pro EZ Gel Solution 10%
JB02-B010S 100ml/BT
EUR 162

T-Pro EZ Gel Solution 12%
JB02-B012M 500ml/BT
EUR 266.4

T-Pro EZ Gel Solution 12%
JB02-B012S 100ml/BT
EUR 162

T-Pro EZ Gel Solution 15%
JB02-B015M 500ml/BT
EUR 266.4

T-Pro EZ Gel Solution 15%
JB02-B015S 100ml/BT
EUR 162

T-Pro Separating or Resolving Buffer
JB03-C001 500ml/BT
EUR 182.4

T-Pro BCA Protein Assay kit
JB04-D001 500assay/KIT
EUR 244.8

T-Pro Transfer Blotting buffer (10X)
JB08-H001 500ml/BT
EUR 162

T-Pro Western Blot Stripping Reagent
JB11-K002 500ml/BT
EUR 213.6

T-Pro Aqua EZ Clean (M)
JT90-R001M 500ml/BT
EUR 398.4

T-Pro Aqua EZ Clean (S)
JT90-R001S 100ml/BT
EUR 181.2

T-Pro EZ stain Gel solution
JT90-R005M 500ml/BT
EUR 224.4

T-Pro EZ stain Gel solution
JT90-R005S 100ml/BT
EUR 151.2

T-Pro P-Fect Transfection Reagent
JT97-N005M 1.0ml/vial
EUR 266.4

T-Pro Plasmid Mini Kit (100)
RB94-YPD100 100preps/Kit
EUR 193.2

T-Pro Plasmid Mini Kit (250)
RB94-YPD250 250preps/Kit
EUR 266.4

T-Pro Plasmid Midi Kit (20)
RB94-YPI020 20preps/Kit
EUR 255.6

T-Pro Plasmid Maxi Kit (10)
RB94-YPM010 10preps/Kit
EUR 266.4

Human Peripheral Blood CD3+ T Cells, Cryopreserved
PBCD3-C10M - Ask for price

Human Peripheral Blood CD3+ T Cells, Cryopreserved
PBCD3-C20M - Ask for price

T-Pro BCA Protein Assay Reagent A
JB04-D001A 500ml/BT
EUR 204

T-Pro BCA Protein Assay Reagent B
JB04-D001B 12ml/BT
EUR 140.4

T-Pro Bradford Protein Assay kit(1X)
JB04-D002 500ml/BT
EUR 193.2

T-Pro Fast Blocking Buffer (in PBS)
JK92-W001 500ml/BT
EUR 224.4

T-Pro Fast Blocking Buffer (in TBS)
JK92-W002 500ml/BT
EUR 224.4

T-Pro Genomic DNA Midi Kit (20)
RB94-NGM020 20preps/kit
EUR 266.4

T-Pro Genomic DNA Mini Kit (100)
RB94-NGS100 100preps/kit
EUR 255.6

PE anti-mouse CD49b (pan-NK cells)
E16FMP049b-050U 50 μg
EUR 433.33
Description: Available in various conjugation types.

APC anti-mouse CD49b (pan-NK cells)
E16FMA049b-025U 25 μg
EUR 303.33
Description: Available in various conjugation types.

T-Pro Laemmli (SDS sample) Reagent (reducing 4X)
JB06-F002 10ml/BT
EUR 151.2

T-Pro Tris-Glycine-SDS running buffer (10X)
JB07-G001 500ml/BT
EUR 162

T-Pro Tris-Glycine-Native running buffer (10X)
JB07-G002 500ml/BT
EUR 162

T-Pro Semi Dry Transfer Blotting buffer (10X)
JB08-H002 500ml/BT
EUR 172.8

T-Pro Protein Free Blocking Buffer (in PBS)
JK92-W003 500ml/BT
EUR 224.4

T-Pro Protein Free Blocking Buffer (in TBS)
JK92-W004 500ml/BT
EUR 224.4

T-Pro Nonliposomal Transfection Reagent I (NTR I)
JT97-N001M 1.0ml/vial
EUR 224.4

T-Pro Nonliposomal Transfection Reagent II (NTR II)
JT97-N002M 1.0ml/vial
EUR 244.8

T-Pro Nonliposomal Transfection Reagent III (NTR III)
JT97-N006M 1.0ml/vial
EUR 266.4

T-Pro Endotoxin Removal Plasmid Midi kit (25)
RB94-EPI020 20preps/Kit
EUR 297.6

T-Pro Endotoxin Removal Plasmid Maxi kit (10)
RB94-EPM010 10preps/Kit
EUR 318

T-Pro Plant Genomic DNA Midi Kit (20)
RB94-PGM020 20preps/kit
EUR 266.4

T-Pro Plant Genomic DNA Mini Kit (100)
RB94-PGS100 100preps/kit
EUR 266.4

Biotin anti-mouse CD49b (pan-NK cells)
E16FMB049b-050U 50 μg
EUR 346.67
Description: Available in various conjugation types.

T-Pro Laemmli (SDS sample) Reagent (non-reducing 4X)
JB06-F003 10ml/BT
EUR 151.2

T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit)
JT96-K002M 250ml*2/Kit
EUR 244.8

T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit)
JT96-K002S 100ml*2/Kit
EUR 182.4

T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit)
JT96-K004M 250ml*2/Kit
EUR 286.8

T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit)
JT96-K004S 100ml*2/Kit
EUR 204

T-Pro Gel/PCR DNA Purification Maxi Kit (10)
RB94-NEL010 10preps/Kit
EUR 193.2

T-Pro Gel/PCR DNA Purification Mini Kit (100)
RB94-NES100 100preps/Kit
EUR 193.2

T-Pro Gel/PCR DNA Purification Mini Kit (250)
RB94-NES250 250preps/Kit
EUR 266.4

T-Pro Washing buffer in PBS and Tween-20 (10X)
JB09-I003 500ml/BT
EUR 172.8

T-Pro Washing buffer in TBS and Tween-20 (10X)
JB09-I004 500ml/BT
EUR 172.8

T-Pro Phosphate-Buffered Saline (PBS, 10X) for western blot washing
JB09-I001 500ml/BT
EUR 162

T-Pro Tris-Buffered Saline (TBS, 10X) for western blot washing
JB09-I002 500ml/BT
EUR 162

PE-Cy7 anti-mouse CD49b (pan-NK cells)
E16FMN049b-025U 25 μg
EUR 411.67
Description: Available in various conjugation types.

PE-Cy7 anti-mouse CD49b (pan-NK cells)
E16FMN049b-100U 100 μg
EUR 975
Description: Available in various conjugation types.

APC-Cy7 anti-mouse CD49b (pan-NK cells)
E16FMO049b-025U 25 μg
EUR 390
Description: Available in various conjugation types.

APC-Cy7 anti-mouse CD49b (pan-NK cells)
E16FMO049b-100U 100 μg
EUR 931.67
Description: Available in various conjugation types.

PerCP-Cy5.5 anti-mouse CD49b (pan-NK cells)
E16FMS049b-025U 25 μg
EUR 520
Description: Available in various conjugation types.

PerCP-Cy5.5 anti-mouse CD49b (pan-NK cells)
E16FMS049b-100U 100 μg
EUR 1278.33
Description: Available in various conjugation types.

Untransduced T Cells
78170-1 1 vial
EUR 1250
Description: The untransduced T cells are produced by mock lentiviral transduction of human primary CD4+CD8+ T cells. These cells are subjected to comparable manipulations as CAR-T cells: activation, spinoculation (without lentivirus), and expansion. These T cells are meant to be negative controls in experiments using lentivirus-transduced primary CAR-T cells.

Untransduced T Cells
78170-2 5 vials
EUR 3325
Description: The untransduced T cells are produced by mock lentiviral transduction of human primary CD4+CD8+ T cells. These cells are subjected to comparable manipulations as CAR-T cells: activation, spinoculation (without lentivirus), and expansion. These T cells are meant to be negative controls in experiments using lentivirus-transduced primary CAR-T cells.

Alexa Fluor® 647 anti-mouse CD49b (pan-NK cells)
E16FMK049b-100U 100 μg
EUR 845
Description: Available in various conjugation types.

CD4+ T cells, Negatively Selected (Human)
79752 10 million cells
EUR 395
Description: Cryopreserved vial (10 x 10^6 cells) of CD4+ T cells that were negatively selected from freshly isolated primary human peripheral blood mononuclear cells (PBMCs). The PBMCs came from a healthy donor, and were isolated from whole blood or leukapheresis samples using a Ficoll gradient. Magnetic antibodies to monocytes, granulocytes, CD8+ T cells, gamma/delta T cells and other immune subsets present in PBMCs were then used to purify untouched CD4+ T cells via immunomagnetic separation. Before and after CD4+ T cell isolation, the cells were stained to evaluate purity and viability by flow cytometry. Cells were cryopreserved in CryoStor CS10 cryopreservation medium (Stemcell, #07930) at a controlled rate._x000D_Source
Normal human PBMC from Leukapheresis Sample

CD8+ T cells, Negatively Selected (Human)
79753 10 million cells
EUR 495
Description: Cryopreserved vial (10 x 10^6 cells) of CD8+ T cells that were negatively selected from freshly isolated primary human peripheral blood mononuclear cells (PBMCs). The PBMCs came from a healthy donor, and were isolated from whole blood or leukapheresis samples using a Ficoll gradient. Magnetic antibodies to monocytes, granulocytes, CD4+ T cells, gamma/delta T cells and other immune subsets present in PBMCs were then used to purify untouched CD8+ T cells via immunomagnetic separation. Before and after CD8+ T cell isolation, the cells were stained to evaluate purity and viability by flow cytometry. Cells were cryopreserved in CryoStor CS10 cryopreservation medium (Stemcell, #07930) at a controlled rate._x000D_Source
Normal human PBMC from Leukapheresis Sample

Rat Pan T cell antibody (biotin)
61R-T135aBT 200 ug
EUR 878.4
Description: Mouse monoclonal Rat Pan T cell antibody (biotin)

Anti-CD19 CAR-T Cells
78171-1 1 vial
EUR 1595
Description: The anti-CD19 CAR-T cells are produced by high-titer lentiviral transduction of human primary CD4+CD8+ T cells using the anti-CD19 CAR Lentivirus (CD19 ScFv-CD8-4-1BB-CD3ζ; SIN Vector, BPS Bioscience, #78601). These ready-to use CAR-T cells express an anti-CD19 CAR consisting the ScFv of CD19 (clone FMC63) linked to a 2nd generation CAR (Chimeric Antigen Receptor) containing CD8 hinge and transmembrane domains, and the 4-1BB and CD3ζ signaling domains. These CAR-T cells have been validated using flow cytometry (to determine the CAR expression) and co-culture cytotoxicity assays.

Anti-CD19 CAR-T Cells
78171-2 5 vials
EUR 4250
Description: The anti-CD19 CAR-T cells are produced by high-titer lentiviral transduction of human primary CD4+CD8+ T cells using the anti-CD19 CAR Lentivirus (CD19 ScFv-CD8-4-1BB-CD3ζ; SIN Vector). These ready-to use CAR-T cells express an anti-CD19 CAR consisting the ScFv of CD19 (clone FMC63) linked to a 2nd generation CAR (Chimeric Antigen Receptor) containing CD8 hinge and transmembrane domains, and the 4-1BB and CD3ζ signaling domains.These CAR-T cells have been validated using flow cytometry (to determine the CAR expression) and co-culture cytotoxicity assays.

Anti-BCMA CAR-T Cells
78660-1 1 vial
EUR 1595
Description: The anti-BCMA CAR-T cells are produced by high-titer lentiviral transduction of human primary CD4+CD8+ T cells using the anti-BCMA CAR Lentivirus (BPS Bioscience #78655). These ready-to-use CAR-T cells express an anti-BCMA CAR consisting of the ScFv of BCMA (clone C11D5.3) linked to a 2nd generation CAR (Chimeric Antigen Receptor) containing CD8 hinge and transmembrane domains, and the 4-1BB and CD3ζ signaling domains (Figure 1). These CAR-T cells have been validated using flow cytometry (to determine the CAR expression) and co-culture cytotoxicity assays.

Anti-BCMA CAR-T Cells
78660-2 5 vials
EUR 4250
Description: The anti-BCMA CAR-T cells are produced by high-titer lentiviral transduction of human primary CD4+CD8+ T cells using the anti-BCMA CAR Lentivirus (BPS Bioscience #78655). These ready-to-use CAR-T cells express an anti-BCMA CAR consisting of the ScFv of BCMA (clone C11D5.3) linked to a 2nd generation CAR (Chimeric Antigen Receptor) containing CD8 hinge and transmembrane domains, and the 4-1BB and CD3ζ signaling domains (Figure 1). These CAR-T cells have been validated using flow cytometry (to determine the CAR expression) and co-culture cytotoxicity assays.

anti-CD4 T helper cells
502-A-01mg 0,1 mg
EUR 321
Description: anti-CD4 T helper cells

anti-CD4 T helper cells
502-A-1000ug 1000 ug
EUR 1539
Description: anti-CD4 T helper cells

anti-CD4 T helper cells
502-A-100ugvial 100 ug/vial
EUR 150
Description: anti-CD4 T helper cells

T-47D Cells Complete Medium
CM-0228-125mL4 125 mL×4
EUR 108
Description: Complete Growth Medium

Human Cord Blood CD8+ Cytotoxic T Cells
CBCD8-C5M 5 million
EUR 958.8

Human Cord Blood CD8+ Cytotoxic T Cells
CBCD8-F5M 5 million
EUR 1078.8

Human Cord Blood CD4+/ CD45RA+ Naive T Cells
CBCD4-45RA-C10M 10 million
EUR 1378.8

Human Cord Blood CD4+/ CD45RA+ Naive T Cells
CBCD4-45RA-C15M 15 million
EUR 1678.8

Human Cord Blood CD4+/ CD45RA+ Naive T Cells
CBCD4-45RA-C5M 5 million
EUR 838.8

Human Cord Blood CD4+/ CD45RA+ Naive T Cells
CBCD4-45RA-F10M 10 million
EUR 1798.8

Human Cord Blood CD4+/ CD45RA+ Naive T Cells
CBCD4-45RA-F15M 15 million
EUR 1980

Human Cord Blood CD4+/ CD45RA+ Naive T Cells
CBCD4-45RA-F5M 5 million
EUR 1198.8

T24 [T-24] Cells Complete Medium
CM-0227-125mL4 125 mL×4
EUR 108
Description: Complete Growth Medium

Albumin Standard (2.0 mg/ml)
JB04-D001C 5*1ml/kit
EUR 162

anti-CD8 T cytotoxic/supressor cells
505-A-01mg 0,1 mg
EUR 321
Description: anti-CD8 T cytotoxic/supressor cells

anti-CD8 T cytotoxic/supressor cells
505-A-1000ug 1000 ug
EUR 1539
Description: anti-CD8 T cytotoxic/supressor cells

Robotic Excessive-Throughput Biomanufacturing and Practical Differentiation of Human Pluripotent Stem Cells

Environment friendly translation of human induced pluripotent stem cells (hiPSCs) is dependent upon implementing scalable cell manufacturing methods that guarantee optimum self-renewal and purposeful differentiation.
Presently, guide tradition of hiPSCs is extremely variable and labor-intensive posing vital challenges for high-throughput purposes. Right here, we established a robotic platform and automatic all important steps of hiPSC tradition and differentiation below chemically outlined situations.
This streamlined method allowed speedy and standardized manufacturing of billions of hiPSCs that may be produced in parallel from as much as 90 totally different patient-and disease-specific cell traces. Furthermore, we established automated multi-lineage differentiation to generate major embryonic germ layers and extra mature phenotypes corresponding to neurons, cardiomyocytes, and hepatocytes.
To validate our method, we rigorously in contrast robotic and guide cell tradition and carried out molecular and purposeful cell characterizations (e.g. bulk tradition and single-cell transcriptomics, mass cytometry, metabolism, electrophysiology, Zika virus experiments) in order to benchmark industrial-scale cell tradition operations in direction of constructing an built-in platform for environment friendly cell manufacturing for illness modeling, drug screening, and cell remedy.
Combining stem cell-based fashions and continuous robotic cell tradition might change into a strong technique to extend scientific rigor and productiveness, that are significantly vital throughout public well being emergencies (e.g. opioid disaster, COVID-19 pandemic).

T-Pro EZ Gel Solution 8%
JB02-B008S 100ml/BT
EUR 162

T-Pro EZ Gel Solution 10%
JB02-B010M 500ml/BT
EUR 266.4

T-Pro EZ Gel Solution 10%
JB02-B010S 100ml/BT
EUR 162

T-Pro EZ Gel Solution 12%
JB02-B012M 500ml/BT
EUR 266.4

T-Pro EZ Gel Solution 12%
JB02-B012S 100ml/BT
EUR 162

T-Pro EZ Gel Solution 15%
JB02-B015M 500ml/BT
EUR 266.4

T-Pro EZ Gel Solution 15%
JB02-B015S 100ml/BT
EUR 162

T-Pro Separating or Resolving Buffer
JB03-C001 500ml/BT
EUR 182.4

T-Pro BCA Protein Assay kit
JB04-D001 500assay/KIT
EUR 244.8

T-Pro Transfer Blotting buffer (10X)
JB08-H001 500ml/BT
EUR 162

T-Pro Western Blot Stripping Reagent
JB11-K002 500ml/BT
EUR 213.6

T-Pro Aqua EZ Clean (M)
JT90-R001M 500ml/BT
EUR 398.4

T-Pro Aqua EZ Clean (S)
JT90-R001S 100ml/BT
EUR 181.2

T-Pro EZ stain Gel solution
JT90-R005M 500ml/BT
EUR 224.4

T-Pro EZ stain Gel solution
JT90-R005S 100ml/BT
EUR 151.2

T-Pro P-Fect Transfection Reagent
JT97-N005M 1.0ml/vial
EUR 266.4

T-Pro Plasmid Mini Kit (100)
RB94-YPD100 100preps/Kit
EUR 193.2

T-Pro Plasmid Mini Kit (250)
RB94-YPD250 250preps/Kit
EUR 266.4

T-Pro Plasmid Midi Kit (20)
RB94-YPI020 20preps/Kit
EUR 255.6

T-Pro Plasmid Maxi Kit (10)
RB94-YPM010 10preps/Kit
EUR 266.4

Human CD3+ T Cells (Pan T Cells), Cryopreserved
M1299-10 each Ask for price

Human CD3+ T Cells (Pan T Cells), Cryopreserved
M1299-20 each Ask for price

Human CD3+ T Cells (Pan T Cells), Cryopreserved
M1299-30 each Ask for price

Human CD3+ T Cells (Pan T Cells), Cryopreserved
M1299-40 each Ask for price

T-Pro BCA Protein Assay Reagent A
JB04-D001A 500ml/BT
EUR 204

T-Pro BCA Protein Assay Reagent B
JB04-D001B 12ml/BT
EUR 140.4

T-Pro Bradford Protein Assay kit(1X)
JB04-D002 500ml/BT
EUR 193.2

T-Pro Fast Blocking Buffer (in PBS)
JK92-W001 500ml/BT
EUR 224.4

T-Pro Fast Blocking Buffer (in TBS)
JK92-W002 500ml/BT
EUR 224.4

T-Pro Genomic DNA Midi Kit (20)
RB94-NGM020 20preps/kit
EUR 266.4

T-Pro Genomic DNA Mini Kit (100)
RB94-NGS100 100preps/kit
EUR 255.6

T-Pro Laemmli (SDS sample) Reagent (reducing 4X)
JB06-F002 10ml/BT
EUR 151.2

T-Pro Tris-Glycine-SDS running buffer (10X)
JB07-G001 500ml/BT
EUR 162

T-Pro Tris-Glycine-Native running buffer (10X)
JB07-G002 500ml/BT
EUR 162

T-Pro Semi Dry Transfer Blotting buffer (10X)
JB08-H002 500ml/BT
EUR 172.8

T-Pro Protein Free Blocking Buffer (in PBS)
JK92-W003 500ml/BT
EUR 224.4

T-Pro Protein Free Blocking Buffer (in TBS)
JK92-W004 500ml/BT
EUR 224.4

T-Pro Nonliposomal Transfection Reagent I (NTR I)
JT97-N001M 1.0ml/vial
EUR 224.4

T-Pro Nonliposomal Transfection Reagent II (NTR II)
JT97-N002M 1.0ml/vial
EUR 244.8

T-Pro Nonliposomal Transfection Reagent III (NTR III)
JT97-N006M 1.0ml/vial
EUR 266.4

T-Pro Endotoxin Removal Plasmid Midi kit (25)
RB94-EPI020 20preps/Kit
EUR 297.6

T-Pro Endotoxin Removal Plasmid Maxi kit (10)
RB94-EPM010 10preps/Kit
EUR 318

T-Pro Plant Genomic DNA Midi Kit (20)
RB94-PGM020 20preps/kit
EUR 266.4

T-Pro Plant Genomic DNA Mini Kit (100)
RB94-PGS100 100preps/kit
EUR 266.4

T-Pro Laemmli (SDS sample) Reagent (non-reducing 4X)
JB06-F003 10ml/BT
EUR 151.2

T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit)
JT96-K002M 250ml*2/Kit
EUR 244.8

T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit)
JT96-K002S 100ml*2/Kit
EUR 182.4

T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit)
JT96-K004M 250ml*2/Kit
EUR 286.8

T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit)
JT96-K004S 100ml*2/Kit
EUR 204

T-Pro Gel/PCR DNA Purification Maxi Kit (10)
RB94-NEL010 10preps/Kit
EUR 193.2

T-Pro Gel/PCR DNA Purification Mini Kit (100)
RB94-NES100 100preps/Kit
EUR 193.2

T-Pro Gel/PCR DNA Purification Mini Kit (250)
RB94-NES250 250preps/Kit
EUR 266.4

T-Pro Washing buffer in PBS and Tween-20 (10X)
JB09-I003 500ml/BT
EUR 172.8

T-Pro Washing buffer in TBS and Tween-20 (10X)
JB09-I004 500ml/BT
EUR 172.8

Human Normal Peripheral Blood CD8+/CD45RA+ Naive Cytotoxic T Cells
PBCD8-45RA-C5M 5 million
EUR 1065.6

Human Normal Peripheral Blood CD8+/CD45RA+ Naive Cytotoxic T Cells
PBCD8-45RA-F5M 5 million
EUR 1318.8

T-Pro Phosphate-Buffered Saline (PBS, 10X) for western blot washing
JB09-I001 500ml/BT
EUR 162

T-Pro Tris-Buffered Saline (TBS, 10X) for western blot washing
JB09-I002 500ml/BT
EUR 162

Immortalized Mouse CD4+ CD8+ T Cells (MOHITO)
T0131 1x106 cells / 1.0 ml Ask for price

Untransduced T Cells
78170-1 1 vial
EUR 1250
Description: The untransduced T cells are produced by mock lentiviral transduction of human primary CD4+CD8+ T cells. These cells are subjected to comparable manipulations as CAR-T cells: activation, spinoculation (without lentivirus), and expansion. These T cells are meant to be negative controls in experiments using lentivirus-transduced primary CAR-T cells.

Untransduced T Cells
78170-2 5 vials
EUR 3325
Description: The untransduced T cells are produced by mock lentiviral transduction of human primary CD4+CD8+ T cells. These cells are subjected to comparable manipulations as CAR-T cells: activation, spinoculation (without lentivirus), and expansion. These T cells are meant to be negative controls in experiments using lentivirus-transduced primary CAR-T cells.

CD4+ T cells, Negatively Selected (Human)
79752 10 million cells
EUR 395
Description: Cryopreserved vial (10 x 10^6 cells) of CD4+ T cells that were negatively selected from freshly isolated primary human peripheral blood mononuclear cells (PBMCs). The PBMCs came from a healthy donor, and were isolated from whole blood or leukapheresis samples using a Ficoll gradient. Magnetic antibodies to monocytes, granulocytes, CD8+ T cells, gamma/delta T cells and other immune subsets present in PBMCs were then used to purify untouched CD4+ T cells via immunomagnetic separation. Before and after CD4+ T cell isolation, the cells were stained to evaluate purity and viability by flow cytometry. Cells were cryopreserved in CryoStor CS10 cryopreservation medium (Stemcell, #07930) at a controlled rate._x000D_Source
Normal human PBMC from Leukapheresis Sample

Anti-CD19 CAR-T Cells
78171-1 1 vial
EUR 1595
Description: The anti-CD19 CAR-T cells are produced by high-titer lentiviral transduction of human primary CD4+CD8+ T cells using the anti-CD19 CAR Lentivirus (CD19 ScFv-CD8-4-1BB-CD3ζ; SIN Vector, BPS Bioscience, #78601). These ready-to use CAR-T cells express an anti-CD19 CAR consisting the ScFv of CD19 (clone FMC63) linked to a 2nd generation CAR (Chimeric Antigen Receptor) containing CD8 hinge and transmembrane domains, and the 4-1BB and CD3ζ signaling domains. These CAR-T cells have been validated using flow cytometry (to determine the CAR expression) and co-culture cytotoxicity assays.

Anti-CD19 CAR-T Cells
78171-2 5 vials
EUR 4250
Description: The anti-CD19 CAR-T cells are produced by high-titer lentiviral transduction of human primary CD4+CD8+ T cells using the anti-CD19 CAR Lentivirus (CD19 ScFv-CD8-4-1BB-CD3ζ; SIN Vector). These ready-to use CAR-T cells express an anti-CD19 CAR consisting the ScFv of CD19 (clone FMC63) linked to a 2nd generation CAR (Chimeric Antigen Receptor) containing CD8 hinge and transmembrane domains, and the 4-1BB and CD3ζ signaling domains.These CAR-T cells have been validated using flow cytometry (to determine the CAR expression) and co-culture cytotoxicity assays.

Anti-BCMA CAR-T Cells
78660-1 1 vial
EUR 1595
Description: The anti-BCMA CAR-T cells are produced by high-titer lentiviral transduction of human primary CD4+CD8+ T cells using the anti-BCMA CAR Lentivirus (BPS Bioscience #78655). These ready-to-use CAR-T cells express an anti-BCMA CAR consisting of the ScFv of BCMA (clone C11D5.3) linked to a 2nd generation CAR (Chimeric Antigen Receptor) containing CD8 hinge and transmembrane domains, and the 4-1BB and CD3ζ signaling domains (Figure 1). These CAR-T cells have been validated using flow cytometry (to determine the CAR expression) and co-culture cytotoxicity assays.

Anti-BCMA CAR-T Cells
78660-2 5 vials
EUR 4250
Description: The anti-BCMA CAR-T cells are produced by high-titer lentiviral transduction of human primary CD4+CD8+ T cells using the anti-BCMA CAR Lentivirus (BPS Bioscience #78655). These ready-to-use CAR-T cells express an anti-BCMA CAR consisting of the ScFv of BCMA (clone C11D5.3) linked to a 2nd generation CAR (Chimeric Antigen Receptor) containing CD8 hinge and transmembrane domains, and the 4-1BB and CD3ζ signaling domains (Figure 1). These CAR-T cells have been validated using flow cytometry (to determine the CAR expression) and co-culture cytotoxicity assays.

anti-CD4 T helper cells
502-A-01mg 0,1 mg
EUR 321
Description: anti-CD4 T helper cells

anti-CD4 T helper cells
502-A-1000ug 1000 ug
EUR 1539
Description: anti-CD4 T helper cells

anti-CD4 T helper cells
502-A-100ugvial 100 ug/vial
EUR 150
Description: anti-CD4 T helper cells

Human Cord Blood CD3+ Pan T Cells
CBCD3-C10M 10 million
EUR 1020

Human Cord Blood CD3+ Pan T Cells
CBCD3-C20M 20 million
EUR 1560

Human Cord Blood CD3+ Pan T Cells
CBCD3-F10M 10 million
EUR 1068

Human Cord Blood CD3+ Pan T Cells
CBCD3-F20M 20 million
EUR 1800

T-47D Cells Complete Medium
CM-0228-125mL4 125 mL×4
EUR 108
Description: Complete Growth Medium

Human Cord Blood CD4+/ CD45RA+ Naive T Cells
CBCD4-45RA-C10M 10 million
EUR 1378.8

Human Cord Blood CD4+/ CD45RA+ Naive T Cells
CBCD4-45RA-C15M 15 million
EUR 1678.8

Human Cord Blood CD4+/ CD45RA+ Naive T Cells
CBCD4-45RA-C5M 5 million
EUR 838.8

Human Cord Blood CD4+/ CD45RA+ Naive T Cells
CBCD4-45RA-F10M 10 million
EUR 1798.8

Human Cord Blood CD4+/ CD45RA+ Naive T Cells
CBCD4-45RA-F15M 15 million
EUR 1980

Human Cord Blood CD4+/ CD45RA+ Naive T Cells
CBCD4-45RA-F5M 5 million
EUR 1198.8

Human Peripheral Blood CD3+ T Cells, Fresh
PBCD3-F20M - Ask for price

Albumin Standard (2.0 mg/ml)
JB04-D001C 5*1ml/kit
EUR 162

T24 [T-24] Cells Complete Medium
CM-0227-125mL4 125 mL×4
EUR 108
Description: Complete Growth Medium

Human Peripheral Blood CD3+ T Cells, Cryopreserved
PBCD3-C10M - Ask for price

Human Peripheral Blood CD3+ T Cells, Cryopreserved
PBCD3-C20M - Ask for price

Human Normal Peripheral Blood CD3+ Pan T Cells
PBCD3-F100M 100 million Ask for price

Human Normal Peripheral Blood CD3+ Pan T Cells
PBCD3-F500M 500 million Ask for price

Edwin

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